|National Collection of Plant Pathogenic Bacteria|
There have been no price rises for NCPPB cultures for several years. Due to increased costs, especially for transport of cultures and to meet the needs of new legislation, we have had to increase our prices.
|Ampoule of freeze dried culture||£75.00 each|
|Packaging, postage and handling charge for UK delivery||£30.00 per order|
|Packaging, postage and handling charge for overseas delivery||£50.00 per order|
|Application for export licence for Australia group organisms for export outside the EU||£POA|
|DNA extracts can be prepared from NCPPB cultures if preferred.|
|Price per extract||£95 + shipping|
|Shipping UK = £30|
|Shipping overseas = £50|
|An Export Licence for DNA from Australia List organisms may be required. See above|
|This method is UKAS accredited and can be regarded as a good method for rapid, accurate and inexpensive identification of bacteria to genus, species or sub-specific rank. Our FAP system is based on the Sherlock Microbial Identification System by MIDI Inc. We require that strains to be identified by FAP are supplied as pure cultures on a simple solid media, such as Nutrient Agar, or on Trypticase Soy Broth Agar (TSBA).|
|2 to 5 isolates||£75.00 per isolate|
|6 to 10 isolates||£41.00 per isolate|
|11 to 20 isolates||£32.00 per isolate|
|21 or more isolates||£27.00 per isolate|
*Fatty acid profiling alone cannot always be relied upon to provide a definitive identification; further tests may be required and we are able to discuss the most appropriate course of action.
|Comparative DNA sequencing analysis is widely considered to be the best genotypic method for microbial identification to species level for many bacteria. The most common approach is PCR amplification and sequencing of all or a 500bp portion of the 16S ribosomal RNA gene. Sequence data is then compared against a sequence database. We can also sequence 'House Keeping' genes such as gyrB, rpoD, and recA. For example, gyrB sequences are sufficiently distinct to allow discrimination of the majority of established species in the genus Xanthomonas. The gyrB phylogeny offers greater resolution amongst Xanthomonads than those produced previously from the 16S rRNA gene or 16S-23S intergenic loci. We would recommend using rpoD for plant pathogenic Pseudomonads and recA for the plant pathogenic Enterobacteriaciae|
|1 isolate||£179.00 per isolate|
|2 isolates||£103.00 per isolate|
|3 isolates||£80.00 per isolate|
|4 isolates||£68.00 per isolate|
|5 isolates||£61.00 per isolate|
|6 to 9 isolates||£57.00 per isolate|
|10 or more isolates||£43.00 per isolate|
|Freeze drying and Deposit services||Please contact NCPPB|
Please note that VAT is to be added where applicable.
Fera will be happy to accept payment by Visa, Mastercard, Switch and Delta. Payment can also be made by cheque, BACS and overseas bank transfer. Please note that cheques must be drawn on a British bank and made payable to 'Food and Environment Research Agency'.