|National Collection of Plant Pathogenic Bacteria|
|CULTURES / IDENTIFICATION AND CHARACTERISATION / TRAINING / CATALOGUE|
|CULTURES Return to top|
|Around 3,500 strains are currently held as lyophilized cultures in glass ampoules, including nearly all type strains of plant pathogenic bacteria. For most we can recommend other typical strains or strains with specific properties. If you wish to work with dead cells and/or cells at a specific concentration we can provide these also. Please contact us to discuss you requirements. We also have a large collection of bacteria stored at -80°C for our own research and for strains that do not freeze dry well. These strains can be supplied as cultures on slants/slopes at the same cost as our ampoules. Please see the price list.|
|WE WILL ONLY TRANSFER MATERIAL TO YOU UNDER THE TERMS AND CONDITIONS OUTLINED IN THE MATERIAL TRANSFER AGREEMENT|
|Click here to view Agreement|
In the UK supply of non-indigenous to UK and non-indigenous to Scotland strains is restricted.
|Depositions of cultures are at the discretion of the Curator. Cultures of pathogenic or research interest or of species that are not well represented in the collection are welcomed. We also accept strains of other taxa that extend their geographical and host ranges or have unusual properties. Authors of new species of bacterial plant pathogens are encouraged to deposit examples in the NCPPB as well as similar specialist collections. The NCPPB accepts reference strains of all newly described taxa.|
|DNA Return to top|
|Crude cell lysates are available, as well as chelex extracted DNA preparations - please contact us to discuss your needs.|
|IDENTIFICATION AND CHARACTERISATION Return to top|
|We offer a rapid identification service based on automated fatty acid profiling*, characterisation methods - including nutritional profiling, and partial gene sequencing.|
|Partial Gene Sequencing|
|Partial gene sequencing can provide accurate identification to the family, genus, species and sub-species level. Using partial gene sequencing and strains from the NCPPB we have carried out a number of studies on the taxonomic relationships within the plant pathogenic xanthomonads and also Pseudomonas syringae and related pathogens for example; comparisons with such studies and published databases allows greater confidence in strain identification. Please contact us for further details.|
|Fatty Acid Profiling/Fatty Acid Methyl Esther analysis (FAP or FAME)|
This method is a rapid, accurate and inexpensive method for the identification of bacteria. We have libraries of profiles for a
wide range of aerobic and anaerobic bacteria of ecological, industrial, medical and agricultural significance. Our FAP system is
based on the Sherlock Microbial Identification System by MIDI Inc. We require that strains to be identified by FAP are supplied as
pure cultures on a simple solid media, such as Nutrient Agar, or on Trypticase Soy Broth Agar (TSBA).
As part of the process involves growing strains for 24 to 48 hours at 28°C it is recommended that for strains that do not grow well under these conditions you contact us to discuss appropriate identification methods and/or a price.
The basic price includes the chromatograph (where available); a profile identifying and quantifying the fatty acids as percentages of the total peak area; one or more library search reports, each with similarity index; histoplots, comparing your values with the library entry for the taxon with the closest fit. If further characterisation tests are required, other than basic nutritional profiling, we will contact you to discuss any extra costs.
* Pest and Disease Diagnostic Services have been accredited by the United Kingdom Accreditation Service to BS/EN/ISO/IEC 17025 General Requirements for the competence of testing and calibration laboratories for tests detailed in the schedule of accreditation number 1642 (available at www.ukas.org (http://www.ukas.org/)